In this research, the mosquitocidal activity of green synthesized silver nanoparticles using five Indian plants Merremia emarginata, Naregamia alata, Hedyotis puberula, Aglaia elaeagnoidea, and Ventilago madrasapatna against three important mosquito vectors Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus were analyzed. Among the five plants aqueous leaf extracts and synthesized silver nanoparticles extract tested, maximum larvicidal, ovicidal, adulticidal activity were observed in Merremia emarginata against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. The biotoxicity of five plant aqueous extract and green-synthesized silver nanoparticles were evaluated on non-target organisms Anisops bouvieri, Diplonychus indicus and Gambusia affinis, Merremia emarginata showed negligible toxicity against these non-target organisms. Suitability index indicated that Merremia emarginata fabricated silver nanoparticles were less toxic to the non-target organism tested if compared to the targeted mosquito larval populations. Therefore, Merremia emarginata fabricated silver nanoparticles could be safely used to control the vectors of mosquitoes in the field applications.